Ammonium ions

b_kanel at bligh.ntu.edu.au b_kanel at bligh.ntu.edu.au
Sun Oct 31 18:17:23 EST 1993


Dear netters,

I have got a problem with the ligation of cDNA into a vector. Unfortunately,
I precipitated the cDNA in the presence of ammonium ions and I suspect that 
the kinase reaction didn't work (Maniatis mentions that ammonium ions 
inhibit polynucleotide kinase).
My question is: does anybody know how to get the ammonium ions out?
I tried precipitation and ethanol washes etc., but it just doesn't help.

Any suggestion would be highly appreciated

Beat



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