In vitro transcriptions

Mr D J Plows lsren at csv.warwick.ac.uk
Wed Sep 1 06:14:15 EST 1993


		Could someone please tell me what I'm doing wrong. When
I run aliquots of my in vitro transcription reaction of a agarose gel, I can
see bands corresponding to the synthesised RNA. However, after 
phenol/chloroforom extraction and ethanol precipitation, the RNA appears
 as a smear on the agarose gel. What's going on or is this normal?

		Thanks in advance

		Dav



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