Etoh wash - confessions of a paranoid researcher....
delisior at rnisd0.DNET.roche.com
Thu Sep 2 12:35:44 EST 1993
"hdang at cns.neusc.bcm.tmc.edu" "Hong Dang" writes:
>>When you do an ethanol wash of a DNA precipitate do you try to resuspend the
>>pellet and spin, rinse the pellet and spin, rinse the pellet and just dry...
>Sometimes it is best to vortex-and-spin. Occasionally, I had high salt
>inhibiting ligation or other enzymes with just rinsing the pellet.
I disagree that vortex-and-spin methods get rid of more salt than simple
difussion. In fact, they always do more harm than good. They shear the DNA and
reprecipitations always lead to sample loss.
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