Etoh wash - confessions of a paranoid researcher....

Bob DeLisio delisior at
Thu Sep 2 12:35:44 EST 1993

"hdang at" "Hong Dang" writes:

>>When you do an ethanol wash of a DNA precipitate do you try to resuspend the
>>pellet and spin, rinse the pellet and spin, rinse the pellet and just dry...

>Sometimes it is best to vortex-and-spin. Occasionally, I had high salt
>inhibiting ligation or other enzymes with just rinsing the pellet.


I disagree that vortex-and-spin methods get rid of more salt than simple
difussion. In fact, they always do more harm than good. They shear the DNA and
reprecipitations always lead to sample loss.


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