Luciferase Assay from Promega
Darin Obrien IHG
darin at gene.med.umn.edu
Fri Sep 3 19:29:59 EST 1993
steven joseph chmura (sjchmura at kimbark.uchicago.edu) wrote:
: Has anyone used this system without the luminometer? The techs claim
: that a scinilation counter can be used effectively.
: Just looking for peoples opinions/experiences. I am trying to
: quantify in-vivo expression.
Yes. We used to do it routinely, however, we have purchased a luminometer recently. See the following for details:
Fulton, R. and Van Ness B. 1993. Luminescent Reporter Gene Assays for
Luciferase and B-Galactosidase Using a Liquid Scintillation Counter.
Biotechniques 14(5), 762-763.
We had to have the scintillation counter equipped with a single photon monitor
to do this. It works well but is not nearly as convenient as a luminometer.
Send questions to fulton at molbio.cbs.umn.edu.
Darin (obrien at molbio.cbs.umn.edu)
ps sorry for the plug, but...
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