Luciferase Assay from Promega

[Darin Obrien IHG]

steven joseph chmura (sjchmura at kimbark.uchicago.edu) wrote:
: Has anyone used this system without the luminometer?  The techs claim
: that a scinilation counter can be used effectively.

: Just looking for peoples opinions/experiences.  I am trying to
: quantify in-vivo expression.



Yes.  We used to do it routinely, however, we have purchased a luminometer recently.  See the following for details:


	Fulton, R. and Van Ness B.  1993. Luminescent Reporter Gene Assays for
Luciferase and B-Galactosidase Using a Liquid Scintillation Counter.
Biotechniques 14(5), 762-763.

We had to have the scintillation counter equipped with a single photon monitor
to do this.  It works well but is not nearly as convenient as a luminometer.  
Send questions to fulton at molbio.cbs.umn.edu.

Darin (obrien at molbio.cbs.umn.edu)

ps  sorry for the plug, but...