Linkers/adapters

Peter M. Muriana muriana at aclcb.purdue.edu
Thu Sep 2 16:12:55 EST 1993


Dear Bionetters,

To those of you who have a lot of experience using adapters/linkers:

We are interested in adding linkers to a bacterial genomic digest, however, 
we do not wish to produce concatamers of genomic fragments, just individual 
fragments which have linkers, or adapters, added to both ends.  Is there 
any other way to prevent concatamerization without resorting to using 
alkaline phosphatase?

Also, is this possibility eliminated by using an excess of linker/adapter 
molecules relative to genomic fragments?

Peter M. Muriana



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