Mutagenesis

Dave Knecht KNECHT at UCONNVM.UCONN.EDU
Wed Sep 8 11:01:48 EST 1993


Hi-  I would like some advice on which approach has been successful for others
to do site directed mutagenesis.  One common method seems to be the Biorad type
 kit that uses the Kunkel selection for uracil containing DNA from the mutageni
zed strand.  The other that seems nice on paper is the Promega kit that uses a
mutagenic oligo to correct a defective amp gene simultaneous with the mutagenes
is of the insert with a second oligo.  Has anyone used the Promega kit succusse
fully and if so what is the efficiency of both oligos working at the same time?
Any comments on these or any other approaches for a lab doing this for the firs
t time.  Dave



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