Cosmid Sequencing-2

Tony Meyn Tmeyn at ccit.arizona.edu
Thu Sep 9 12:34:43 EST 1993


I am trying to use cosmids for templates in sequencing rxns with mixed 
results.  So far the best sequence has been generated using DNA that has
been treated with T7 gene 6 exonuclease.  Does anyone have experience with
this
enzyme or its use in sequencing?  How about any other ideas that might help
get more sequence from comids?

Tony Meyn
University of Arizona
TMeyn at ccit.arizona.edu



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