"Hole" in the middle of Southern bands?
sre at al.cam.ac.uk
Fri Sep 10 02:07:31 EST 1993
In article <CD3HA8.Ez9 at news.cis.umn.edu> horton at molbio.cbs.umn.edu (Robert Horton) writes:
>Randy Hardy (hardy at mighty.fccc.edu) wrote:
>: Here's a weird problem: recently we have started seeing empty centers to
>: intense bands in our PCR-Southerns (amplify, blot, probe with internal
>: fragment, autorad). Previous months of blotting never showed this...
>Yes, my colleague recently got some glorious doughnut bands. He used standard
>capillary transfer with paper towels, on to a Nytran membrane. I'd like to
>hear explainations for this, too :)...
Bubbles between the gel and the membrane during transfer, I'd bet.
Not too weird.
- Sean Eddy
- Laboratory of Molecular Biology, MRC, Cambridge UK
- sre at mrc-lmb.cam.ac.uk
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