"Hole" in the middle of Southern bands?

[Sean Eddy]

In article <CD3HA8.Ez9 at news.cis.umn.edu> horton at molbio.cbs.umn.edu (Robert Horton) writes:
  >Randy Hardy (hardy at mighty.fccc.edu) wrote:
  >:  Here's a weird problem: recently we have started seeing empty centers to
  >: intense bands in our PCR-Southerns (amplify, blot, probe with internal
  >: fragment, autorad).  Previous months of blotting never showed this...
  >Yes, my colleague recently got some glorious doughnut bands. He used standard
  >capillary transfer with paper towels, on to a Nytran membrane. I'd like to
  >hear explainations for this, too :)...

Bubbles between the gel and the membrane during transfer, I'd bet.
Not too weird.






--
- Sean Eddy
- Laboratory of Molecular Biology, MRC, Cambridge UK
- sre at mrc-lmb.cam.ac.uk