Plasmid mini-prep purification for in vitro translation?
preissj at clvax1.msu.edu
Fri Sep 10 13:41:00 EST 1993
In Article <Pine.3.07.9309091414.A8373-a100000 at hamlet.ucdavis.edu> "szsclark at HAMLET.UCDAVIS.EDU (Sonya Clark)" says:
> ... in vitro transcriptions and translations ... We have found that
> CsCl-purified plasmid will give the most efficient incorporations.
> Standard mini-prepped plasmid DNA doesn't work. (is there) a good
> easy method that gives really good transcription/translation rates?...
I haven't done any in vitro transcription yet, but I've been thinking about
doing some in the future. Hence I am also very interested in the answer. I
will add that I have heard rumors that DNA purified by Quiagen collumn works
very well for this purpose. Does anyone out there have any experience with
Dr. Leonard N. Bloksberg
More information about the Methods