bisbenzimide CsCl gradients

mcgraw at BSCR.UGA.EDU mcgraw at BSCR.UGA.EDU
Fri Sep 10 16:26:47 EST 1993


Hello all,

We're trying to isolate an AT-rich fraction of DNA from the rest.  We read
that equilibrium density gradient centrifugation in the presence of 
bisbenzimide should work for this.  We tried it a couple of times with
no luck yet.  I figure we're making some kind of very basic mistake, but
we haven't figured it out yet.  So.  Is there someone out there who has
a lot of experience with this method?  We're not even seeing bands of any kind,
main or AT-rich at this point.  What are they supposed to look like?

Any comments, either to me directly or to the net, would be greatly
appreciated.  Thanks.

Al McGraw
mcgraw at bscf.uga.edu



More information about the Methods mailing list