Cloning large fragments

Ali Karami ali at biobase.aau.dk
Fri Sep 10 06:05:58 EST 1993


In <2653rb$8k0 at max.physics.sunysb.edu> kramer at mcbsgi.bio.sunysb.edu (joe kramer) writes:

>Hello all;

>   I need to make a multistep construction that approaches 
>30 kb.  I was wondering what hints,  help, and warnings
>y'all might have concerning such an undertaking.  Some
>of the questions I have are as follows:  

>Are pUC based vectors somewhat limited in their 
>ability to carry large segments...Is pBR better
>or is there something else.

>Are certain bacterial strains more or less disposed 
>for the task?

>Are there any tricks and rules-of-thumb concerning 
>ligations involving large inserts (20kb?)

>Thanks.
>Joe Kramer
>   

I think  Cosmids are more suitable fore cloning of large fragments.

 ali



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