Cloning large fragments
ali at biobase.aau.dk
Fri Sep 10 06:05:58 EST 1993
In <2653rb$8k0 at max.physics.sunysb.edu> kramer at mcbsgi.bio.sunysb.edu (joe kramer) writes:
> I need to make a multistep construction that approaches
>30 kb. I was wondering what hints, help, and warnings
>y'all might have concerning such an undertaking. Some
>of the questions I have are as follows:
>Are pUC based vectors somewhat limited in their
>ability to carry large segments...Is pBR better
>or is there something else.
>Are certain bacterial strains more or less disposed
>for the task?
>Are there any tricks and rules-of-thumb concerning
>ligations involving large inserts (20kb?)
I think Cosmids are more suitable fore cloning of large fragments.
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