Cosmid Sequencing-2

Martin Leach leach at mbcrr.harvard.edu
Mon Sep 13 08:22:00 EST 1993


In article <Tmeyn-090993102211 at blast.agmarley.arizona.edu>,
Tmeyn at ccit.arizona.edu (Tony Meyn) wrote:

> I am trying to use cosmids for templates in sequencing rxns with mixed 
> results.  So far the best sequence has been generated using DNA that has
> been treated with T7 gene 6 exonuclease.  Does anyone have experience with
> this
> enzyme or its use in sequencing?  How about any other ideas that might help
> get more sequence from comids?
> 
> Tony Meyn
> University of Arizona
> TMeyn at ccit.arizona.edu


Why not try digesting them with a rare cutting r. enz.
linearized templates will sequence more easy
and less problems with steric effects and all that

Martin Leach

leach at mbcrr.harvard.edu



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