SP6 polymerase on PCR products?

Jose A. Godoy jagodoy at mendel.berkeley.edu
Mon Sep 13 20:58:23 EST 1993


Hi, there! I would like to use SP6 polymerase on PCR products to generate
riboprobes but I was discouraged by the report of Logel et al
(Biotechniques 13(4): 604-610, 1992). Using PCR products as templates, they
successfully synthesize cRNA with T7 or T3 but get no or very little
incorporation with SP6. Has anybody used SP6 successfully on these kind of
templates? I would appreciate any comments or suggestions.



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