colony hybs

Ludwig Lab-UCSC niflab at biology.ucsc.edu
Mon Sep 13 16:36:20 EST 1993


>I'm planning to screen my clones with bumblebee DNA in order to find
>microsatellites. I'm going to use a radiactively marked probe and
>hybridize on some kind of filter. Does anyone have a protocol on how
>to get the bacteria on to the filter? What kind of filter shall I use?
>Nylon or nitrocellulose ones?
>
>Thanks in advance,
>
>Peter Thoren

I use a microwave oven (really) and paper filters (Whatman 540 or 541
are stiff enough).  It's very quick and gives good signal-to-noise.
Forster et al. Trends in Genetics 6:141 (1990).

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	Robert Kuhn, PhD
	Sinsheimer Laboratories
	University of California
	Santa Cruz, CA 95064  USA
      
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	email: rkuhn at orchid.ucsc.edu
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