Testing for mycoplasma contamination
mkennedy at chmeds.ac.nz
Wed Sep 15 19:06:57 EST 1993
I have a question about mycoplasma testing of mammalian cell cultures. I grow
mouse ES cells, and it is critical to avoid mycoplasma contamination if these
cells are to be returned to developing mouse embryos.
Does anyone have information about PCR tests for a range of common mycoplasma?
I understand that amplification of rRNA species, or something similar, can be
diagnostic for these things. Ideally, I would like sequences for a pair of
primers that are able to identify common mycoplasma contaminants in mammalian
I know about Hoechst staining methods, culture methods, and the GenProbe TC
rapid detection system that uses nucleic acid hybridization with a tritiated
probe, and I see that Stratagene also now sell primers for Mycoplasma PCR
Any comments, information would be greatly appreciated, either by posting or
by e-mail and I'll summarize for the net.
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-750
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