Luciferase spectrum

Mark Mattson mpm at seqanal.mi.uky.edu
Thu Sep 16 09:57:13 EST 1993


Steve Barger (mpm at seqanal.mi.uky.edu) wrote:
: Does anyone know of literature concerning the emission spectrum of luciferin
: generated by firefly luciferase?  What I really need to know is whether these
: emissions will interfere with fura-2 fluorescence.

: Thanks,
: Steven W. Barger, Ph.D.
: Sanders-Brown Center on Aging

I have now answered my own question.  In case anyone's interested, emission
max is at 562 at pH 7.1-7.6 according to Seliger & McElroy, Arch Biochem
Biophys 88: 136.  Emission at 500 is almost negligible.

Steve



More information about the Methods mailing list