Luciferase spectrum

Mark Mattson mpm at
Thu Sep 16 09:57:13 EST 1993

Steve Barger (mpm at wrote:
: Does anyone know of literature concerning the emission spectrum of luciferin
: generated by firefly luciferase?  What I really need to know is whether these
: emissions will interfere with fura-2 fluorescence.

: Thanks,
: Steven W. Barger, Ph.D.
: Sanders-Brown Center on Aging

I have now answered my own question.  In case anyone's interested, emission
max is at 562 at pH 7.1-7.6 according to Seliger & McElroy, Arch Biochem
Biophys 88: 136.  Emission at 500 is almost negligible.


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