Midi-preps

Martin Leach leach at mbcrr.harvard.edu
Mon Sep 20 06:30:48 EST 1993


In article <20SEP93.09282530.0062 at VM1.MCGILL.CA>, BGAA at MUSICB.MCGILL.CA
(BGAA) wrote:

> As the title implies, does anyone know of a good midi-prep protocol
> that is fast and inexpensive?  I recently tried one that was
> published in an April '93 issue of Biotechniques where the authors
> suggested using 40% PEG in 30mM MgCl2 to purify the plasmid DNA.
> However, everything worked for me until I got to this step and then
> I lost my DNA pellet.  I suspect that the concentration of PEG
> is too high and doesn't fractionate the DNA very well.  Any
> suggestions?
> 
> 
> Tony Kwan
> Department of Biochemistry, McGill University
> BGAA at MUSICB.MCGILL.CA


Hi,

We use Qiagen tip-500 columns

1-800-426-85

the affinity columns are a little pricey
but you can get Transfection pure plasmid DNA after approx. 4 hrs. (most of
this is spin time).

Martin Leach



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