Purification of ss-DNA from PCR

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Mon Sep 20 16:21:28 EST 1993

In article <CDI5xw.C2y at ncifcrf.gov> drummond at ncifcrf.gov
 (James Drummond) writes:

> I am looking for a way to purify my PCR products into their
> respective ssDNA.  Does anyone know of a method which will allow
> me to do this?

What purpose did you have in mind? If sequencing or using only one strand as a
probe, you could use a 5' biotinylated primer on one side during the PCR.
Afterward, bind the PCR product to magnetic particles and strip off the ssDNA
using NaOH. There's a protocol in the Promega book listed in the FAQ.

* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*

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