sequencing lambda clone

Bruce Elder elder at CGL.UCSF.EDU
Mon Sep 20 18:06:24 EST 1993


First of all, thanks to those who responded so promptly to my first posting
about "directly" sesquencing a lambda clone. However, I must apologize
because my wording was a bit unclear.  What I want to do is take a lambda
clone and use it as template for double strand sequencing. The primers
I refered to are sequencing primers to one end of the clone. I cant
do
conventional PCR first because I dont have any 3' sequence, although this
would be a good way as people suggested. Sorry for  the misunderstanding
and does anyone have any suggestions now?

Bruce Elder
elder at cgl.ucsf.edu



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