Primers database through gopher.

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Mon Sep 20 17:18:22 EST 1993


In article <1993Sep20.173226.23593 at aristo.tau.ac.il>
 pc386 at ccsg.tau.ac.il (BENNY SHOMER 9238) writes:

> It has been recently suggested that a searchable database of primers 
> for PCR will be set.

> Sequence (5' --> 3'): ___________________________     
> Length: __
> Flanking bases ______ to _____ on the target sequence. 

***> Tm of primer and based on which method?

***> Computer program used to select primer?

***> Reason for selecting primer in this location? (sometimes I like
to include or exclude a natural restriction site for later manipulation
although the primer may not be the "best" match for the amplification)

> **********************   Buffer Constituents  ****************************
>
> (This section basically regards non-standard buffer constituents)
>
> Reaction Volume (ul): __
>
> Used Standard Buffer Supplied By: _______________
>
>
> MgCl2 Concentration (mM): __      
> DMSO Concentration (%): __ 
>
> Formamide Concentration (%): __   
> Gelatin Concentration (%): __ 

***> Other additives used?

> dNTP's Concentration (uM): __     
> Primers Concentration (uM): __ 
>
> Polymerase Type: ___________   
> Polymerase Concentration (U/Rxn.): ___ 
>
> Make: _____________
  ^^^^
***> I assume this is the make and model of the PCR machine? 

*******************************************************************************
* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
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