E. coli/Bacillus shuttle vectors
danj at dev.gdb.org
Tue Sep 21 11:05:52 EST 1993
In article <01H30O8G8N0Y0000EZ at nic.the.net>, SHAUN%JASON.DECNET at relay.the.net ("Shaun D. Black") writes:
> We are working on expression of a gene in E. coli, with good results. But,
> it has become of interest to express the same gene in Bacillus subtilis.
> Thus, we have two questions: 1. Does anyone have a preference for any of
> the E. coli/Bacillus shuttle vectors out there?, and 2. Will an E. coli
> promoter work in Bacillus? Advice and/or references most appreciated.
Point your gopher client at gopher.gdb.org and select:
--> 12. Search Databases at Hopkins (Vectors, Promoters, NRL-3D, EST, OMIM../
--> 6. Culture Collections (ATCC, ECACC)/
--> 1. ATCC - The American Type Culture Collection/
--> 7. Recombinant DNA Materials (Clones, Vectors, Libraries, Hosts, Olig../
--> 2. Vectors <?>
And search for:
subtilis and coli and shuttle
You'll find 14 or so vectors that may be of interest.
Best of luck,
danj at mail.gdb.org
More information about the Methods