Silent ClaI sites - info ?

tom_chappell at tom_chappell at
Thu Sep 23 09:51:50 EST 1993

In article <jjw-230993123321 at> jjw at (Jeremy
Weinman) writes:
>Silent ClaI sites
>Does anybody out there in netland know of any info (published or
>on why ClaI sometimes doesn't recognize a ClaI site?  We've got some
>which we have been mapping and we have a cla site that cuts on the basis
>some unknown or perhaps archane variable, seemingly regardless of how
>other cla sites in the clone are cutting.  Any thoughts?

dam methylation? in a random sequence 1 in 8 Cla sites will have a GA in
front or a TC after and be dam methylated. Cla does not cut methylated

tom chappell

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