Silent ClaI sites - info ?

tom_chappell at cellbio.duke.edu tom_chappell at cellbio.duke.edu
Thu Sep 23 09:51:50 EST 1993


In article <jjw-230993123321 at 150.203.39.67> jjw at rsbs1.anu.edu.au (Jeremy
Weinman) writes:
>Silent ClaI sites
>
>Does anybody out there in netland know of any info (published or
otherwise)
>on why ClaI sometimes doesn't recognize a ClaI site?  We've got some
clones
>which we have been mapping and we have a cla site that cuts on the basis
of
>some unknown or perhaps archane variable, seemingly regardless of how
well
>other cla sites in the clone are cutting.  Any thoughts?
>=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
=-=-=

dam methylation? in a random sequence 1 in 8 Cla sites will have a GA in
front or a TC after and be dam methylated. Cla does not cut methylated
sites.

tom chappell



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