Lambda restriction problem

kavousi at mpimg-berlin-dahlem.mpg.de kavousi at mpimg-berlin-dahlem.mpg.de
Thu Sep 23 10:23:59 EST 1993


Dear Netters,

I am trying to isolate a 1.4 kb fragment from a Lambda gt11 DNA, using the
enzyme EcoRI but under normal condition I get very small amount of restricted
fragment. It seems that the DNA is not commpletely restricted with EcoRI.
Has anyone any idea how to improve the completeness of restriction?

Thanks for any suggestion

			Mehdi Kavousi

Kavousi at MPIMG-Berlin-Dahlem.MPG.DE



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