Microwave mini-preps

Bruce Roe broe at aardvark.ucs.uoknor.edu
Sat Sep 25 09:54:00 EST 1993


In article <23SEP93.15397427.0070 at VM1.MCGILL.CA>, BGAA at MUSICB.MCGILL.CA (BGAA) writes...
>My boss told me that when he was back doing his post-doc a while ago,
>someone was microwaving E.coli and extracting the plasmid DNA.  He
>did it really quick and the quality was very good for sequencing.
>However, this person did not give the entire protocol to my boss and
>so he never got it to work himself.
> 
>Does anyone know of this technique or something similar?
> 
>Tony Kwan
>Dept. of Biochemistry, McGill University
>BGAA at MUSICB.MCGILL.CA

Tony,
	The September 1993 (vol15, No.3) issue of Biotechniques contains
two articles which may be relevelant:

	D. C. Goodman and S. B. Lee, Microwave Miniprep of Total Genomic
 DNA from Fungi, Plants, Protists. Biotechniques 15, 438-444 (1993)

	A. K. Meeker, Y-K. Li, D. Shortle, and W. E. Stites, A Simplified
 Protocol for Isolation and Characterization of ssM13 DNA Templates for Use
 in Dideoxy Sequencing. Biotechniques 15, 370-372 (1993).

	Has anyone given them a try?

Cheers.....bruce
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