Ligation-med. PCR: the "C" reaction
mst at seqanal.mi.uky.edu
Mon Sep 27 15:28:13 EST 1993
I was wondering if anyone who does ligation-mediated PCR has any
suggestions on getting the Maxam-Gilbert "C" reaction to work. I
work with genomic DNA (from tissue culture cells) and I can get the
"G" reaction to work most of the time. I would assume that there is
an impurity in the DNA that affects the hydrazine, but not the DMS.
Thanks to anyone who can help.
Peggy L. Bishop Internet address is: mst at seqanal.mi.uky.edu
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