Ligation-med. PCR: the "C" reaction

Mitch Turker mst at seqanal.mi.uky.edu
Mon Sep 27 15:28:13 EST 1993


I was wondering if anyone who does ligation-mediated PCR has any
suggestions on getting the Maxam-Gilbert "C" reaction to work.  I
work with genomic DNA (from tissue culture cells) and I can get the 
"G" reaction to work most of the time.  I would assume that there is
an impurity in the DNA that affects the hydrazine, but not the DMS.
Thanks to anyone  who can help.
  
Peggy L. Bishop   Internet address is:  mst at seqanal.mi.uky.edu



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