Sensitive Westerns.

FOF at FDACFSAN.BITNET FOF at FDACFSAN.BITNET
Tue Sep 28 08:19:12 EST 1993


Regarding the need for extra-sensitive Westerns: I once had to overcome a
similar problem.  I ended up enriching the protein by denaturing immunopr-
ecipitations and then running it on a gel and Western blotting.  If your
protein is much over 70 kD there should be no problem with the IP Ab
interfering. Oh yeh, I used chemiluminescence detection.



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