Ligation-med. PCR: the "C" reaction

Vivian Miao vmiao at
Tue Sep 28 22:45:11 EST 1993

In article <CE14v3.B8p at>, mst at (Mitch Turker)
> I was wondering if anyone who does ligation-mediated PCR has any
> suggestions on getting the Maxam-Gilbert "C" reaction to work.  I
> work with genomic DNA (from tissue culture cells) and I can get the 
> "G" reaction to work most of the time.  I would assume that there is
> an impurity in the DNA that affects the hydrazine, but not the DMS.
> Peggy L. Bishop   Internet address is:  mst at

Do you know that it is the genomic DNA that is the problem, and not the
hydrazine itself?  (e.g. tested it on some nice clean cloned DNA)?

More information about the Methods mailing list