Extracting Rat DNA

Jim Cummins cummins at possum.murdoch.edu.au
Wed Sep 29 15:42:18 EST 1993


We are having problems extracting DNA from rat tissues using the D NA
PrepAGene matrix kit from Australian Biosearch.  Tissues are
homogenised with 1% SDS lysis buffer and proteinase K and incubated at
56C for 3 hours.  We had some succes earlier with prior extraction
using chloroform/phenol, but the prep was a bit dirty.
Anybody got any good ideas?

Jim Cummins                   
School of Veterinary Studies
Murdoch University
Western Australia 6150  Tel +61-9-360 2668 Fax +61-9-310 4144
For every complex problem there's a simple solution.  And it's wrong!



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