Extracting Rat DNA
cummins at possum.murdoch.edu.au
Wed Sep 29 15:42:18 EST 1993
We are having problems extracting DNA from rat tissues using the D NA
PrepAGene matrix kit from Australian Biosearch. Tissues are
homogenised with 1% SDS lysis buffer and proteinase K and incubated at
56C for 3 hours. We had some succes earlier with prior extraction
using chloroform/phenol, but the prep was a bit dirty.
Anybody got any good ideas?
School of Veterinary Studies
Western Australia 6150 Tel +61-9-360 2668 Fax +61-9-310 4144
For every complex problem there's a simple solution. And it's wrong!
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