fast oligo size fractionation

APPLING at UTBC01.CM.UTEXAS.EDU APPLING at UTBC01.CM.UTEXAS.EDU
Wed Sep 29 12:01:13 EST 1993


For years we have used thin layer chromatography to size-fractionate and
purify deprotected oligos.  See Alvarado-Urbana et al: Science 214:270-274,
1981 for the method.  We use plates containing fluorescent indicator from
EM Science (# 5715-7, silica gel 60F-254) and develop them in N-propanol/
NH4OH/H2O (55:35:10).  This technique is simple and good for oligos up to
about 25 nt.

_______________________________________________________________________________
Dean R. Appling
Dept. of Chemistry & Biochemistry
Univ. of Texas at Austin
Austin, TX 78712
(512)471-5842
(512)471-5849 (FAX)
appling at utbc01.cm.utexas.edu
_______________________________________________________________________________




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