ligation

MWB6 at psuvm.psu.edu MWB6 at psuvm.psu.edu
Wed Sep 29 15:43:56 EST 1993


question about ligation: I assume it is best tthat the DNA to be ligated is
supplied in TE or H2O. I have a small fragment of 200 bp obtained by pcr and
cut by EcoR1. CAn i use an aliquot of this DNA (dissolved in reaction3 Gibco
buffer) for ligation directly instead of purifying the dna first. Which ions
or buffer components do inhibit ligation strongly?
thanx for any reply
michael



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