Promoter sequences T3 / SP6

[Paul Riggs]

In article <289bv3$7mr at BKFUXG.kfunigraz.ac.at>,
koraiman at bedvgw.kfunigraz.ac.at (Dr. G. Koraimann) wrote:
> 
> Hi bionetters!
> Can anyone help me with sequences that are sufficient for T3 or SP6
> RNA polymerases to work? What are the minimal promoters for these
> enzymes to work in in vitro transcription assays ?
> Thanks
> Gnther Koraimann 

Here's an answer relayed from Chris Noren (noren at neb.com)

T3: -17 AATTAACCCTCACTAAAG +1

T7: -17 ATTTAGGTGACACTATAG +!

If you need any more info, you can contact him directly.
Paul
______
Paul Riggs
New England Biolabs
riggs at neb.com