Partial Protein Digests NTCB?

bss032 at uk.ac.bangor.vaxa bss032 at uk.ac.bangor.vaxa
Thu Sep 30 10:56:08 EST 1993


Does anyone know what NTCB and IBzO are in the context of protein cleavage
reagents? Also, are there any tricks to the digestion of proteins with
ProEn a proline specific protease? I tried the standard 100 mM phosphate
buffer recommended with no cleavage whatsoever over a range of concs.
Any cofactor requirements? etc
Thanks in advance.

Jon R Sayers (Cell and Molecular Biology), Biochemistry Dept.,
University College North Wales, Univ of Wales, Bangor, LL57 2UW.
Tel +44 248 38 23 54
Fax +44 248 370731
Email BSS032 at BANGOR.AC.UK



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