Methods September 1993 Archives by date

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Starting: Wed Sep 1 01:22:08 EST 1993
Ending: Thu Sep 30 23:23:16 EST 1993
Messages: 575

BioTechniques subscription Sebastian Bunka
MaConkeys agar/Xgal Greg Denomme
5'UTR of eukaryotic mRNA GWDGV1::JHANES
Etoh wash - confessions of a paranoid researcher.... David Johnstondaj
MaConkeys agar/Xgal GRGGTA at uk.AC.MANCHESTER.CHRISTIE-HOSPITAL.PATERSON
In vitro transcriptions Mr D J Plows
Pfu Polymerase Questions Bruce Roe
minigels on slides keep floating away - help K.C. Baker
5'UTR of eukaryotic mRNA John E. Blume
Etoh wash - confessions of a paranoid researcher.... John Nash
HELP! Ligation of tomato genomic DNA Bill Melchior
phenol/chloroform extraction Bill Melchior
Metabolic activity dye? Anthony Davies
Etoh wash Bill Melchior
Etoh wash - confessions of a paranoid researcher.... Hong Dang
Primer preparation K.C. Baker
why Ag stained DNA bands are brown? John Hartmann
PCR - amplification Paul N Hengen
Pulse-Chase Protocols Antje Haase
Etoh wash - confessions of a paranoid researcher.... Michael G. Kurilla
Pfu Polymerase Questions Michael G. Kurilla
turboblotter nishir at ohsu.edu
PRIMER (was: Re: Pfu Polymerase Questions) Toby Bradshaw
Etoh wash - confessions of a paranoid researcher.... Basavaraju Shankarappa
turboblotter Glenda A. Baker
European Collection of Animal Cell Cultures (ECACC) via Gopher Dan Jacobson
codon freq C. fasciculata John Mazzella
codon freq C. fasciculata Ananias Escalante
Fermentation Instrumentation Martin Rink
Problem with a genomic Southern blot Sonya Clark
Pfu Polymerase Questions Sheila Gay Buzzee
HELP: Template for Sequenase not clean enough Andrew McArthur
DNA extraction needed for Chestnut Gus Trautweiler
In vitro transcriptions Matthew Thomas
PCR Program Kevin Morano
HELP: Template for Sequenase not clean enough srgcles at grv.grace.cri.nz
"Crud" in plasmid preparation Michael Coady
Etoh wash DAVIS at licre.ludwig.edu.au
ANTHEPROT: A package for protein sequence analysis G.Delage
ABI 373A automated sequencer - logistics of use/experience David Johnstondaj
Antibodies to nuclear proteins Janet L. Marcucci
Luciferase Assay from Promega steven joseph chmura
Cloning large fragments joe kramer
PCR Program Michael G. Kurilla
Etoh wash - confessions of a paranoid researcher.... Bob DeLisio
ligation of tomato dna john hachey
Linkers/adapters Peter M. Muriana
clogged DNA synthizer lines Jouko Kalevi Pettersson
runoff/run-on Ludwig Lab-UCSC
Saran wrap Martti Tolvanen
Etoh wash - confessions of a paranoid researcher.... DAVIS at licre.ludwig.edu.au
BioTechNet GRGGTA at uk.AC.MANCHESTER.CHRISTIE-HOSPITAL.PATERSON
Packaging extracts Antonio R. Franco
Saran wrap roger at kean.ucs.mun.ca
Agrobacterium minipreps? JON BOXALL.
Who sells 96-well PCR machines? K.C. Baker
Saran wrap Robert Preston
Saran wrap GIETZ at bldghsc.lan1.umanitoba.ca
18S/28S RNA in kb douglas gray cancer research
Saran wrap afc at gnv2
ABI 373A automated sequencer - logistics of use/experience Bruce Roe
Direct Sequencing of Chromosomal DNA Rodney Tweten
Lipid isolation with CTAB ??? Jutta Reidlinger
Commercial source for sequencing gels? pathology at mscf.med.upenn.edu
****Chicken Library(3rd attempt)**** Hirdeypal S. Bhathal
ABI 373A automated sequencer - logistics of use/experience Bob DeLisio
cDNA library Yuri
18S/28S RNA in kb Andre Hamel
18S/28S RNA in kb DAVID F. BISHOP
ABI 373A automated sequencer - logistics of use/experience John H McDonald
Luciferase Assay from Promega Darin Obrien IHG
Saran wrap Lawrence P. Casson
Competent Cell Prep jbhogen at merle.acns.nwu.edu
Packaging extracts Duncan Clark
18S/28S RNA in kb Andre Hamel
Recycle QIAprep columns? Juliette Faraco
MiniProtean II and 2D gels Michael J Marino
18S/28S RNA in kb Mitchell F. Balish
18S/28S RNA in kb Mitchell F. Balish
Direct Sequencing of Chromosomal DNA ww40
Saran wrap David Johnstondaj
Adaptors against linkers Antonio R. Franco
anybody knows how to contact American Diagnostica ? Massimo Delledonne
Poltergeist in Serratia marcescens mrege at cnbvx3.cnb.uam.es
Saran wrap croth at pasteur.fr
Poltergeist in Serratia marcescens M.J. Horsfall
HELP! Ligation of tomato genomic DNA Leonard Bloksberg
Digitiser for the Mac? parkhillj at vms1.bham.ac.uk
Luciferase Assay from Promega Leonard Bloksberg
Saran wrap Leonard Bloksberg
Packaging extracts Leonard Bloksberg
Agrobacterium minipreps? Leonard Bloksberg
anything that kills bacteria but not yeast? Michael Levin
Nylon Wool fergusond at uk.ac.afrc
differential display TECCE at IT.CSATA.MVX36
Saran wrap John Hartmann
which is YOUR favorite vacuum blotter? Dave Segal
anything that kills bacteria but not yeast? Francis Ouellette
HELP change enz storage buffer? Dave Segal
Packaging extracts Duncan Clark
anything that kills bacteria but not yeast? RPSCOTT
HELP change enz storage buffer? Karl Fischer
lambda gt 22 primers jreich at fr.u-strasbg.illite
DNA SEQUENCING Dr. M.D. Jones
SUMMARY -Logistics of running an ABI 373A sequencing facility David Johnstondaj
HELP: Beta-strand (sheet) prediction!!! willemsen
differential display VAN BELZEN at PA1
actinobacillus library yvind Johnsen
Commercial source for sequencing gels? Jim Owens
Saran wrap Martti Tolvanen
Digitiser for the Mac? Amy Teel
actinobacillus library Brain Foley
anything that kills bacteria but not yeast? Dubear Kroening
Digitiser for the Mac? David Stillman
Recycle QIAprep columns? Brian Halligan
Poltergeist in S. marcescens strain mrege at cnbvx3.cnb.uam.es
HELP: Beta-strand (sheet) prediction!!! Robert Horton
anything that kills bacteria but not yeast? Jonathan Greene
test Marc Gwadz
Help: His6X proteins Marc Gwadz
Competent Cell Prep Antje.Haase at RT1.MENZIES.SU.EDU.AU
help tomato nuclear extract Tao Wei
****Chicken Library(3rd attempt)**** nishir at ohsu.edu
Use MacConkey plate to screen M13 recombinants? Zhixiang Hu
antibody to neomycine phosphotransferase? Josef Peter Magyar
in situs in parafin-embeded tissue using digoxigenin CHALK at gr.forth.imbb.nefelh
MURINE C-SRC CDNA (NON-NEURONAL) CLONE Dr. M.D. Jones
Commercial source for sequencing gels? Jim Owens
runoff/run-on Ken Krauter
in situs in parafin BERMAN02 at swmed.edu
Mutagenesis Dave Knecht
Etoh wash - confessions of a paranoid researcher.... Paul N Hengen
mosquitoe probe request RANGEL at CINVESMX.BITNET
Mutagenesis William R. Morgan
of Seran Wrap and M&M's Leonard Bloksberg
Mutagenesis Leonard Bloksberg
runoff/run-on Leonard Bloksberg
anything that kills bacteria but not yeast? Mark Mattson
transfer of dsRNA to nitrocellulose Morag Lancaster
anything that kills bacteria but not yeast chai_z at wehi.edu.au
anybody knows how to contact American Diagnostica ? William R. Morgan
Digitiser for the Mac? William R. Morgan
Use MacConkey plate to screen M13 recombinants? Russell A. Maurer
anything that kills bacteria but not yeast? Jeffrey Wong
of Seran Wrap and M&M's Klaus.Matthaei at anu.edu.au
Mutagenesis parkhillj at vms1.bham.ac.uk
DNA Sequencing Services Anne Howe
"Hole" in the middle of Southern bands? Randy Hardy
anything that kills bacteria but not yeast? Marci Swede
Luciferase Assay from Promega Tao Tao
"Hole" in the middle of Southern bands? Robert Horton
Cosmid Sequencing-2 Tony Meyn
deuterated detergents Michael Eisen
materials help Greg Beck
Regulatory genes (not really methods) Peter J. Russell
Plasmid mini-prep purification for in vitro translation? Sonya Clark
PCR of seed samples rpl at tower
Genomic Southern Hybridization Questions chai_z at wehi.edu.au
Sequence Data Submissions Dennis Benson
anything that kills bacteria but not yeast? Michael Benedik
"Hole" in the middle of Southern bands? Sean Eddy
How do you get clean template DNA? michael_m at vcp1.vcp.monash.edu.au
EMBL3 map rdkortschak at science.adelaide.edu.au
Cloning large fragments Ali Karami
anything that kills bacteria but not yeast? Andre B.
Lead Acetate Counterstain? Philip Fortgens
hole in the middle of southern bands ? suter at de.d400.mpg.MPIZ-KOELN.VAX
Direct Sequencing of Chromosomal DNA-additional info Rodney Tweten
Genomic Southern Hybridization Questions Eleanor Gallo-Hendrikx
Anti-HIV-1 Gag p15? Anthony Davies
anything that kills bacteria but not yeast? BERMAN02 at swmed.edu
Urea Gradient Gel Devices Michael Ready - or a host of others
Autoradiography: "Hole in the middle of Southern Bands" pmiguel at bilbo.bio.purdue.edu
Epitope tagging Zimanyi Esteban
Genomic Southern Hybridization Questions wetsel_r at msgcg.wustl.edu
Single Stranded DNA Sequencing John Cooper
Fotodyne Gene Sprinter greg at MENDEL.LLNL.GOV
Plasmid mini-prep purification for in vitro translation? Leonard Bloksberg
Single Stranded DNA Sequencing BERMAN02 at swmed.edu
Autoradiography: "Hole in the middle of Southern Bands" Johan de Boer
Genomic Southern Hybridization Questions Leonard Bloksberg
"Hole" in the middle of Southern bands? Jim Gerlach
seed-storage protein promoter Parin
PCR in plant genomic DNA CHAIVISU at UCONNVM.bitnet
bisbenzimide CsCl gradients mcgraw at BSCR.UGA.EDU
Restriction Mapping saast8 at vms.cis.pitt.edu
"Hole" in the middle of Southern bands? Antje Haase
PCR in plant genomic DNA Wolfgang Wegloehner
hole in the middle of southern bands (? reciprocity failure) Cheung C. Yue
NET.BIO.NET OFFLINE FOR 12 hours TODAY, 9/11 Dave Kristofferson
anything that kills bacteria but not yeast? Ron Emaus
DNA Sequencing Services Bruce Roe
anything that kills bacteria but not yeast? Ron Emaus
hole in the middle of southern bands (? reciprocity failure) Martin Leach
Cosmid Sequencing-2 Martin Leach
Luciferase Assay from Promega Martin Leach
PCR primers and hybridization probes ljc55 at cas.org
Gene Hunter Corporation address kanyuka at uk.ac.afrc
of Seran Wrap and M&M's(candy!!) dallas at mcvax4.d48.lilly.com
"Hole" in the middle of Southern bands?(More for less) dallas at mcvax4.d48.lilly.com
Extra bands C-myc Northern blots? Michael Dews
Summary: TA cloning vector RICHARD P. PHIPPS
GenHunter corp. Address USA::LLR8156
colony hybs Ludwig Lab-UCSC
5'race XIN at UNCVX1.OIT.UNC.EDU
**URGENT: pUC118 VECTOR SEQUENCE NEEDED** Peter J. Russell
Urea Gradient Gel Devices neale at mbcf.stjude.org
Generating Very HOT Riboprobes Roger F. Anderson
SP6 polymerase on PCR products? Jose A. Godoy
Single Stranded DNA Sequencing William R. Morgan
dam- lambda host? rdkortschak at science.adelaide.edu.au
Nested deletions with double stranded plasmids Dr. A. Rosenthal
Nested deletions with double stranded plasmids William R. Morgan
Detecting Biotinylated PCR products? Jim Gerlach
Mitochondrial volume rogersh at uk.ac.afrc
oligo-affinity chromatography YUNJ at UNCVX1.OIT.UNC.EDU
Use of Salt Gradient in Sequencing Gels? Robert Rumpf
antibody to diphtheria toxin DAVID FRISCH
Ligating large constructs David J. Kroll
PCR of seed samples Mark Schweder
PCR in plant genomic DNA Mark Schweder
Use of Salt Gradient in Sequencing Gels? Michael Finney
Single Stranded DNA Sequencing the End
posting a message Los Dmitry Anatolievitch
PCR Jyothi Sookram
IFAGE for sizing bacterial genomes Darren Martin
Saran wrap Tony Hodge
Birch cDNA library TIMO KERVINEN MMKAB_OPISK
Competent Cell Prep Tony Hodge
multiple bands on GAPDH S. blot Darin Obrien IHG
MacTetrad - Excel spreadsheet for yeast genetics David Stillman
PCR in single cell Parin
Lots of GAPDH retropseudogenes in mammals John Logsdon
help with ExoIII deletions Andrew Tai
Source of TNF-alpha probe? PECHAN at HELIX.MGH.HARVARD.EDU
Help in cDNA library construction Alexandra Nava Kalof
Single Stranded DNA Sequencing the End
PCR Dirk Bornemeier
GUS assays in E. coli JON BOXALL.
Phosphor Imager - update Jonathan B. Marder
Help with acid-phenol extraction biolc4 at jane.uh.edu
Availability of and antibody against Coxiella burnetti. Brian Robertson
Ligating large constructs Dr. A. Rosenthal
Testing for mycoplasma contamination Martin Kennedy
Source of TNF-alpha probe? Martin Kennedy
multiple bands on GAPDH S. blot Klaus.Matthaei at anu.edu.au
Who sells 96 well PCR machines (summary) K.C. Baker
Luciferase spectrum Mark Mattson
Luciferase spectrum Mark Mattson
bisbenzimide CsCl gradients Leonard Bloksberg
Nested deletions with double stranded plasmids Leonard Bloksberg
Use of Salt Gradient in Sequencing Gels? Pamela McLean
What's the Magic in Magic Minipreps? ed beaty
Does the sequence file for pBSKS/pBSSk exist? Paul Kowalski
FAQlist for bionet.molbio.methds-reagnts Paul N Hengen
BIOSYS-1 program LEES at SASK.USASK.CA
ExoIII is a Dinosaur. Try DNAse 1 or TN1000 Roger F. Anderson
E. coli/Bacillus shuttle vectors Shaun D. Black
Does the sequence file for pBSKS/pBSSk exist? Francis Ouellette
What's the Magic in Magic Minipreps? Paul N Hengen
Single Stranded DNA Sequencing jrbrown at ac.dal.ca
FIGE bands Ludwig Lab-UCSC
FIGE Ludwig Lab-UCSC
colony hybs Martin Leach
Ligating large constructs Bruce Roe
Summary: TA cloning vector Martin Leach
E. coli/Bacillus shuttle vectors dallas at mcvax4.d48.lilly.com
Purification of ss-DNA from PCR James Drummond
HELP, need address of Pfalz & Bauer Charles E. Weaver
USBs email address Charles E. Weaver
UV irradiation and exon splicing FRANK,BART
Lymphoblast plating question gebowd at woods.ulowell.edu
anything that kills bacteria but not yeast? Chris Black
SP6 polymerase on PCR products? Dan Kolk
yeast vectors Jose_Costoya
just a test James Drummond
archives? What archives? Christopher Carlson
acryl-agarose gels fred at bmt.mcw.edu
IBI E-mail address Matthew Wakefield
Promega Magic Mix Dr. C.J. Chan
Reverse transcription JONG, SONG-MUH J
YEAST EXPRESSION LIBRARY rlawson at wesleyan.edu
T phage? ralston at ROCKVAX.ROCKEFELLER.EDU
USBs email address Duncan Clark
2-D Gel Streaking and/or Gel "Sandwiches" Problems mills_j at mscf.med.upenn.edu
T phage? Sean Eddy
Gopher Access to the Bionet Archives (Long) Bruce Roe
T phage? Patricia Foster
horizontal IEF fred at bmt.mcw.edu
Extra Bands in in vitro transcription/primer extension Daniel Mytelka
colony hybs Martin Kennedy
multiple bands on GAPDH S. blot Andrew Hobbs
IMPORTANT BIOSCI INFORMATION David Kristofferson
impedimetric monitoring of bacterial growth huyberechts at imecom.imec.be
T phage? R.G. Walters
Midi-preps Martin Leach
Midi-preps BGAA
IBI E-mail address Martin Leach
Summary-lamda exo ssDNA prep awalley at molbiol.ox.ac.uk
acryl-agarose gels Michael van Waes
help: ligation xho1 problems Michael G. Kurilla
help: ligation xho1 problems suter at de.d400.mpg.MPIZ-KOELN.VAX
ligation problems suter at de.d400.mpg.MPIZ-KOELN.VAX
direct sequenceing of lambda clone Bruce Elder
Primers database through gopher. BENNY SHOMER 9238
Ligating large constructs neale at mbcf.stjude.org
Supershift of bZIP protein The Fish Speaker
ligation problems GIETZ at bldghsc.lan1.umanitoba.ca
Oligonucleotides for Microsatellites Dave Harry
Primers database through gopher. Andrew Joseph Kompanek
Primers database through gopher. Andrew Joseph Kompanek
direct sequenceing of lambda clone Dave Harry
pdb files to secondary structures ? flo at UNIXG.UBC.CA
E. coli/Bacillus shuttle vectors Shaun D. Black
T phage? Russell A. Maurer
Purification of ss-DNA from PCR Paul N Hengen
Midi-preps nishir at ohsu.edu
Screening large fragment fro gchen at kean.ucs.mun.ca
"Hole" in the middle of Southern bands?(More for less) Euan R. Taylor
Primers database through gopher. Paul N Hengen
sequencing lambda clone Bruce Elder
Reuse of Large and expensive Qiagen columns john peloquin
Holes in intense southern bands. FOF at FDACFSAN.BITNET
sequencing lambda clone Martin Kennedy
Screening large fargment fro gchen at kean.ucs.mun.ca
Oligonucleotides for Microsatellites William R. Morgan
direct sequenceing of lambda clone William R. Morgan
E-MAIL FOR ABI ??? Donna Munoz O'Regan
tRNA to use as carrier Mary Metzler, Plants, x8076
Faster oligo size-fractionation? Graham Threadgill
Primers database through gopher. Roland Gronroos
Primers database through gopher. Keith Hutchison
Cancellation of E-mail BRYANT at swmed.edu
Extraction Procedure:-Critique Carl Weatherell
Odd bacterial phenomenon Mary Metzler, Plants, x8076
exon splicing and cell stress info needed FRANK,BART
xgpt activity Mats Nilsson
cosmid prep info wanted lsalati at wvnvms.wvnet.edu
antibody to diphtheria toxin Dan Jacobson
E. coli/Bacillus shuttle vectors Dan Jacobson
Midi-preps Leonard Bloksberg
help: ligation xho1 problems Leonard Bloksberg
Screening large fargment fro Leonard Bloksberg
T phage? Duncan Clark
Primers database through gopher Luc Simon
MH1
Midi-preps Bill Melchior
E-MAIL FOR ABI ??? WAGNERF at BRUNB.BITNET
Hi! Promega monitors! Roger F. Anderson
spermidine was Re: Ligat'g lg constructs nishir at ohsu.edu
Degenerate Primers John Nash
Primers database through gopher. Michael Finney
xgpt activity Christine Gee
T phage? RYBICKI, ED
T phage? Jarmo Niemi
exoIII is a dinosaur.try dnase1 or tn1 suter at de.d400.mpg.MPIZ-KOELN.VAX
Cm-Toyopearl superfine column ... where ? Massimo Delledonne
tRNA to use as carrier Bruce Roe
cosmid prep info wanted Bruce Roe
Phosphor Imager - update Mike Morris
E-MAIL FOR ABI ??? Jaime Prilusky
E-MAIL FOR ABI ??? Jaime Prilusky
Kontes pestels Larry Hale
Beckman ultra tube heat sealer Darin Obrien IHG
RNA structure prediction APPLING at UTBC01.CM.UTEXAS.EDU
cosmids etc rogersh at uk.ac.afrc
Midi-preps Bernard Heymann
Kontes pestels Michael Coady
Reuse of Large and expensive Qiagen columns Chris Upton
Degenerate Primers Karl A. Suiter
To all UK PCRers bss032 at uk.ac.bangor.vaxa
Primers database through gopher nishir at ohsu.edu
Midi-preps Jorge Blackhall
Gel imagers fzestabr at bullwinkle.ucdavis.edu
T phage? Michael Finney
Midi-preps Antje.Haase at RT1.MENZIES.SU.EDU.AU
Midi-preps Michael Coyne
To all UK PCRers txpljfg at UABCVSR.CVSR.UAB.EDU
Degenerate Primers ww40
T phage? Michael Coady
T phage? CHALK at gr.forth.imbb.myia
Alkaline Phosphatase Michael Coady
phosphorimagers afc at gnv.ifas.ufl.edu
Request for Editing Patricia Foster
Alkaline Phosphatase Inactivation RPSCOTT
Kontes pestels Karl Fischer
Silent ClaI sites - info ? Jeremy Weinman
help: ligation xho1 problems Andy McShea
Request for Editing Rosemary Hoffman
Midi-preps Michael Benedik
T phage? Michael Benedik
Silent ClaI sites - info ? Michael Benedik
How to lower background of silver staining Sebastian Bunka
yeast viability assay help IBELGAUFTS at de.mpg.biochem.vms
To all UK PCRers David Johnstondaj
Cm-Toyopearl superfine column ... where ? Christopher Cilley
turbo screening? Martin Leach
To all UK PCRers Mr. D.J. Atherton
Silent ClaI sites - info ? vernon
How to lower background of silver staining Dirk Bornemeier
Silent ClaI sites - info ?: Dam-Methylase again knoblauch at mpimg-berlin-dahlem.mpg.de
Silent ClaI sites - info ? tom_chappell at cellbio.duke.edu
Lambda restriction problem kavousi at mpimg-berlin-dahlem.mpg.de
xhoI cloning problems suter at de.d400.mpg.MPIZ-KOELN.VAX
Cm-Toyopearl superfine column ... where ? Jonathan B. Marder
Preping samples for SDS-PAGE Dan Roberts
turbo screening? Alex Borovkov
Degenerate Primers JOHNSON_T
Capillary Electrophoresis on ABI 270A-HT David Martin x3175
Beckman ultra tube heat sealer Peter M. Muriana
Silent ClaI sites - info ? Peter M. Muriana
Address of Indofine Fine Chemicals? Amanda Coutts
How to lower background of silver staining Huang Bixing
ultrasonic ligation reqiest Alex Borovkov
To all UK PCRers Michael Finney
T phage? Michael G. Kurilla
Midi-preps Michael G. Kurilla
Silent ClaI sites - info ? Michael G. Kurilla
help: ligation xho1 problems Duncan Clark
Microwave mini-preps BGAA
Reuse of Large and expensive Qiagen columns Duncan Clark
More on: Primers database / gopher. BENNY SHOMER 9238
5-methylcytosine quantitation Mike Muszynski
5-methylcytosine quantitation Mike Muszynski
USBs email address Parke K. Flick
To all UK PCRers Duncan Clark
DNA and RNA isolation Bob Steagall
Expression Vector SMITH_DENNIS_P at LILLY.COM
Answer for microwave mini-preps and Xho1 WANGNN at UCBEH.SAN.UC.EDU
DNA and RNA isolation nishir at ohsu.edu
To all UK PCRers Duncan Clark
RNA structure prediction the End
33P gel info? JOHN LATIMER
Sequence For pNL43 Needed Len Adleman
Primers database through gopher. Brain Foley
Thymoll Questions Helen McBride
Reuse of Large and expensive Qiagen TRIGGS at bldghsc.lan1.umanitoba.ca
Not1 DIGESTS AND FIGE GELS. ROLF PRADE
DNA and RNA isolation Basavaraju Shankarappa
Genomic DNA Prep from Heparinized blood? Robert Wildin
USBs email address Michael Coady
Microwave mini-preps Dan Maloney
Turbo screening Ludwig Lab-UCSC
turbo screening? RYBICKI, ED
More on: Primers database / gopher. vernon
rna fingerprinting help schuchert at urz.unibas.ch
Kaback hybridization Ely Rabani
Re Protocol for Turbo-lifting/screening Martin Leach
ultrasonic ligation reqiest Bruce Roe
T phage? Amy Teel
Antibody to T7 Gene 10 Protein? Philip L. Carl
RNA fingerprinting ? Noway, it is garbage! WANGNN at UCBEH.SAN.UC.EDU
RNA LAB INC Dr. M.D. Jones
Lumino meters Reon Brand
T phage? MYGALP01 at ulkyvm.louisville.edu
yeast viability assay help Kathie Hodge
Cleaning up membranes. FOF at FDACFSAN.BITNET
Ligation of ~20kb fragment into pUC119/pUC118 Angelo Gunasekera
SDS Removal from proteins Carl Weatherell
Gopher Access to the Bionet Archives (Long) Geraldine A. New
ligation of ~20kb fragment into pUC118/pUC119 Angelo Gunasekera
ligation of ~20kb fragment into pUC118/pUC119 Angelo Gunasekera
receptor expression Jose_Costoya
Problem of horizontal polyacrylamide gel electrophoresis Huang Bixing
ExoIII is a Dinosaur. Try DNAse 1 or TN1000 Roger Anderson
Silent ClaI sites - info ? pmiguel at bilbo.bio.purdue.edu
Could 3'-UTR reduce expression level? Zheng-Tao Shi
Microwave mini-preps Bruce Roe
DNA -> RNA ..... steven joseph chmura
Trays (Sequencing Gels) ez002976 at othello.ucdavis.edu
Microwave mini-preps the End
More on: Primers database / gopher. BENNY SHOMER 9238
ECL and binding. BENNY SHOMER 9238
Trays (Sequencing Gels) Bruce Roe
Trays (Sequencing Gels) Martin Leach
Lumino meters Martin Leach
Cells dying in serum-free medium John Boswell
More on: Primers database / gopher. BENNY SHOMER 9238
Antibody to T7 Gene 10 Philip L. Carl
Where can get CEPH DNA, help! Mu Jianlong
Old Techniques Wanted(for DNA Labeling, Immobilization, Denaturing) Roger Bryner
DNA -> RNA ..... RPSCOTT
DNA -> RNA ..... RPSCOTT
testing for mycoplasma contamination Martin Kennedy
Cells dying in serum-free medium macdouga at wren.sunnybrook.utoronto.ca
Accommodation in Bethesda michael_m at vcp1.vcp.monash.edu.au
Any good reviews for gel-shift analyses. Martin Leach
Cells dying in serum-free medium RYBICKI, ED
Faster oligo size-fractionation? BERMAN02 at swmed.edu
Western Blotting brwgor01 at uctvax.uct.ac.za
E-MAIL FOR ABI ??? Bob DeLisio
Factor Xa or Thrombin restriction site batu erman
Faster oligo size-fractionation? David Martin x3175
DNA -> RNA ..... the End
Could 3'-UTR reduce expression level? Paul Riggs
Cells dying in serum-free medium nishir at ohsu.edu
Ligation-med. PCR: the "C" reaction Mitch Turker
Cells dying in serum-free medium Antje Haase
DNA -> RNA ..... Martin Kennedy
Degenerate Primers vernon
Looking for Whatman's address LEES at SASK.USASK.CA
cloning biotinylated DNA's Jim Gale
pdb files to secondary ...Summary gilkes at unixg.ubc.ca
polyanion heparin Byung-hoon Kim
help: ligation xho1 problems Byung-hoon Kim
purification of an unstable mutant Zheng-Tao Shi
Cells dying in serum-free medium RYBICKI, ED
"Negative Staining" on Western Blots Ronald Berry
Looking for Whatman's address Martin Leach
Promoter sequences T3 / SP6 Dr. G. Koraimann
Ligation-mediated PCR. FOF at FDACFSAN.BITNET
Sensitive Westerns. FOF at FDACFSAN.BITNET
Unstable mutant enzyme. FOF at FDACFSAN.BITNET
"Negative Staining" on Western Blots HYDE at horta
Antibody to T7 Gene 10 Philip L. Carl
T phage? Summary (long) ralston at ROCKVAX.ROCKEFELLER.EDU
Unstable DNA spacers Brian Halligan
Degenerate Primers Paul N Hengen
Western Blotting nishir at ohsu.edu
Degenerate Primers John Nash
polyanion heparin Diana Ralston
pulse-chase references Mark Watson
IBI Robert Coelen
IMPORTANT - NEW TIBS/BIOSCI PROJECT!! Dave Kristofferson
PCR in single cell from plant parin
Inosine in PCR? Dr. A. Rosenthal
Human Cell Viability Assay? CX74
Lambda restriction problem sarven
looking for DNA Strider William Gomes
Inosine in PCR? Vivian Miao
In situ transcription/T7 amplification sarven
Ligation-mediated PCR. Vivian Miao
Ligation-med. PCR: the "C" reaction Vivian Miao
Human Cell Viability Assay? Andy McShea
IEF gels Sarah Tilley
Who has phage P1? Johan de Boer
looking for DNA Strider pmartino at pasteur.fr
Affinity purification of protein malaine at sara.cc.utu.fi
Inosine in PCR?(deazaGTP) dallas at mcvax4.d48.lilly.com
Use of 33-P in blots USA::AWS4270
Protocol for E. coli. northern Yang Tang
"Negative Staining" on Western Blots nishir at ohsu.edu
Extracting Rat DNA nishir at ohsu.edu
Factor Xa or Thrombin restriction site Anthony Davies
fast oligo size fractionation APPLING at UTBC01.CM.UTEXAS.EDU
Inosine in PCR?(deazaGTP) Vivian Miao
Promoter sequences T3 / SP6 Paul Riggs
invitro extracts Matthew Coleman
Promega's Silver Sequencing kit, opinions wanted. mrege at cnbvx3.cnb.uam.es
Extracting Rat DNA Jim Cummins
ligation MWB6 at psuvm.psu.edu
ligation MWB6 at psuvm.psu.edu
assay for tannic acid scarr at kean.ucs.mun.ca
Who has testicular cDNA libraries? Michael Holland
xhoI cloning problems-additions Jay Rothstein
Molecular Biologist/protein chemist (Ph.D) seeking a position Kunapuli T. Madhusudhan
Inosine in PCR? Klaus.Matthaei at anu.edu.au
Human Cell Viability Assay? Robert M. Straubinger
Sterilization of materials bob prohaska
SEEKING phage T4 gene 43 44/62 and 45 proteins DAVE SEGAL
Human Cell Viability Assay? Michael J Conboy
Imagene from Molecular Probes Reon Brand
el340 ultra-kinetics ELISA reader bio-tec instruments? IBELGAUFTS at de.mpg.biochem.vms
Aspen vs Pine bedding for lab animals "PRASAD S. KULKARNI"
ligation Brain Foley
Partial Protein Digests NTCB? bss032 at uk.ac.bangor.vaxa
transfer proteins from a stained gel CMSMUELLER at MINNA.ACC.IIT.EDU
anti-double strand DNA antibody (wanted) UMT601 at rhrz.uni-bonn.de
Software for the Mac GIETZ at bldghsc.lan1.umanitoba.ca
anti-double strand DNA antibody (wanted) dLee
POST DOC Laurel Arthur
Cells dying in serum-free medium macdouga at jay.sunnybrook.utoronto.ca
Cheap Xgal HRMBDKC at HKUCC.HKU.HK
Cells dying in serum-free medium mills_j at mscf.med.upenn.edu
Gel Drying Infuriation mills_j at mscf.med.upenn.edu

Last message date: Thu Sep 30 23:23:16 EST 1993
Archived on: Sun Mar 6 19:54:52 EST 2005

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