5' biotin labeling of oligos

Shiao Y. Wang sywang at whale.st.usm.edu
Fri Apr 1 12:40:23 EST 1994


Steve Rodems (smrodems at students.wisc.edu) wrote:
: In article <2nbv4f$15r at usenet.INS.CWRU.Edu>, ch994 at cleveland.Freenet.Edu
: (Parke K. Flick) wrote:

: > 
: > Glad you asked, Liam.  USB has just introduced the Oligonucleotide
: > Biotin Labeling Kit which labels standard oligos after they have
: > been made.  In conjunction with the SurePure tlc system from USB,
: > you can label and purify an oligo in about 4 hours.  By the way,
: > you can also label with fluorescein at the 5' end by analogous
: > reactions.
: > 					P. Flick

: How sensitive are these labels?  Could they be used in gel shift
: experiments?  Also, how stable are they?  One bad thing about 32P is that
: you probe is useless after a couple weeks.

More questions:
Are the oligos stable for PCR? If so, will the biotin hang on long enough
for detection or purification via one of the avidin-biotin methods? Thanks.



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