Cleaning seq. plates with "Bon Ami"??

Dan Diaz bl275 at cleveland.Freenet.Edu
Fri Apr 1 15:24:26 EST 1994


In a previous article, sywang at whale.st.usm.edu (Shiao Y. Wang) says:

>Thanks for the tip. One question: what is the purpose of soaking the new
>glass plates in 2M NaOH? Won't the glass be etched or is that the intended
>purpose so that acrylamide will stick better? 

a one hour soak wont etch the glass much, but it does remove invisible gunk
left on the plates after manufacture.  if you do this once, you wont have
to repeat it.  if you follow the procedure i outlined, a simple rinse with
water followed by brief cleaning with glass plus and isopropanol will get
you going - no need for extensive harsh cleansers.

diz



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