Site directed mutagenesis
aiyo at uoguelph.ca
Fri Apr 1 23:27:06 EST 1994
I have finished sequencing a cellulase gene and the translated
sequence when aligned with some other cellulases in the same family show
some "putative" conserved residues in the family. I have been thinking of
what next to do with this sequence and I thought changing some of the so
called conserved residues might be fun. The idea is to see how these
changes would affect certain characteristics of the enzyme.
So I am looking for a relatively straight forward method I could
use to alter some of these residues. I had read a paper in which they
used "a unique restiction enzyme site elimination technique" with a kit
from Clonetech laboratories. The method seemed very straight forward but
I thought I should just ask in case someone out there may have used it.
If yes could you just give me some insights ? And if there are other
simpler methods one can use I'd welcome any suggestions.
You my want to send replies to me. My email address
is aiyo at uoguelph.ca
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