Sequencing gel apparatus suggestions

Sean David Moore smoore at sas.upenn.edu
Mon Apr 4 09:30:08 EST 1994


I thnk it may be that you are not sealing the plates against the comb as
it sets.  This will couse a "mushy" edge where the acrylamide doesn't
fully polymerize (contact with air) so when you put the comb in, the teeth
set onto a soft suface.  You could try more clamps at the top of the gel
 and across the top as it sets.

SEAN MOORE
smoore at sas.upenn.edu
baldy at brahms.udel.edu
TATABOX at aol.com




More information about the Methods mailing list