Immunoscreen needed

Mr. Steven Swift; BIOL; GRAD sswift at umbc.edu
Tue Apr 5 07:55:39 EST 1994


	I am working in S. cerevisiae and an looking for a protocol for
  immunoscreening mutagenized yeast colonies. I am looking for the presence
  of a specific plasma membrane protein (epitope tagged) for which I have 
  antibody against. I have seen some protocols (nitrocellulose/Ab) for
  E.coli but I need one for yeast colonies.
	Please E-mail me any yeast immunoscreening protocols or references
  you might have.

	sswift at umbc7.umbc.edu

	Thanks in advance,
			   Steve


--


>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
STEVE SWIFT - My opinions are my own and may not be shared by others, TOUGH! 
<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
"Stupidity is its own reward" - SS, "Shit Happens" - someone else
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
"Tell me something I don't know! 
 Tell me something, something I can use!
 Push the button, connect the Goddamm DOTS!" - Ministry
<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<



More information about the Methods mailing list