Sequencing Gel adheres to both plates

Wasun Chantratita asmsi002 at CMU.CHIANGMAI.AC.TH
Wed Apr 6 00:04:59 EST 1994


Dear Netters,

	We are now working on nonradioactive, silver DNA sequencing
system. Our gel must be firmly stick to the short plate and not peel off
during silver staining process. Our problem is the adhering of
polyacrulamide gel oftenly occur to both plates.  Perhaps our long glass
plate might be contaminated with Bind-silane from the short plate.  We do
wipe excessive binding solution (Bind-silane) from the short plate, we do
apply Sigmacote solution on the long plate, we also clean our plates with
Windex cleaner. However, this problem still repeatedly strike us. Is 
there anyone out there could help me to solve this problem? 

	Any suggestion would be deeply appreciated.
 
Thank you,

Wasun

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Wasun Chantratita, Ph.D.                        Phone:053-221122 Ext 5086,5068
Department of Clinical Microbiology             Fax:  053-221890 
Faculty of Associated Medical Sciences       Email:asmsi002 at cmu.chiangmai.ac.th
Chiang Mai University
Chiang Mai 50200
Thailand
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