dextran sulfate-- a cheaper source??

Steven Coon at umich.edu
Wed Apr 6 15:18:31 EST 1994


In article <Cnt28w.IuG at news.cis.umn.edu>, mman at molbio.cbs.umn.edu (Michael
Man) wrote:

>  	I use dextran sulfate in my hybridization solution.  It gives me very 
> good results, but it is too expensive, $732 per 500 gms from Sigma.  Does 
> anyone know a cheaper place to go?  Thanks for your help.
> 	Also I will be very grateful if you can suggest an alternative way 
> to prepare prehyb. and hyb. solution.  I use GeneScreen transfer membrane and
> UV-crosslink RNA. 
> 
> 
> Michael
> mman at molbio.cbs.umn.edu


Sorry but no matter where you go Dex sulfate is expensive. Try using less
solution by putting your blots into sealable bags. Solution are pretty
standard too and I certainly wouldn't change it especially if what you are
doing works.

Steve Coon
sdcoon at umich.edu



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