dextran sulfate-- a cheaper source??

Steven Coon at
Wed Apr 6 15:18:31 EST 1994

In article <Cnt28w.IuG at>, mman at (Michael
Man) wrote:

>  	I use dextran sulfate in my hybridization solution.  It gives me very 
> good results, but it is too expensive, $732 per 500 gms from Sigma.  Does 
> anyone know a cheaper place to go?  Thanks for your help.
> 	Also I will be very grateful if you can suggest an alternative way 
> to prepare prehyb. and hyb. solution.  I use GeneScreen transfer membrane and
> UV-crosslink RNA. 
> Michael
> mman at

Sorry but no matter where you go Dex sulfate is expensive. Try using less
solution by putting your blots into sealable bags. Solution are pretty
standard too and I certainly wouldn't change it especially if what you are
doing works.

Steve Coon
sdcoon at

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