dextran sulfate-- a cheaper source??
Wed Apr 6 15:18:31 EST 1994
In article <Cnt28w.IuG at news.cis.umn.edu>, mman at molbio.cbs.umn.edu (Michael
> I use dextran sulfate in my hybridization solution. It gives me very
> good results, but it is too expensive, $732 per 500 gms from Sigma. Does
> anyone know a cheaper place to go? Thanks for your help.
> Also I will be very grateful if you can suggest an alternative way
> to prepare prehyb. and hyb. solution. I use GeneScreen transfer membrane and
> UV-crosslink RNA.
> mman at molbio.cbs.umn.edu
Sorry but no matter where you go Dex sulfate is expensive. Try using less
solution by putting your blots into sealable bags. Solution are pretty
standard too and I certainly wouldn't change it especially if what you are
sdcoon at umich.edu
More information about the Methods