dcarpenter at receptor.pharm.uwa.edu.au
Wed Apr 6 03:18:59 EST 1994
Can anyone with any experience in percoll gradients and their use in
fractionation of subcellular compartments please help me with protocols
which work! I plan to use pancreatic, hepatic and T cell lines to
primarily identify endosomes, lysosomes and plasma membranes. The protocols
I have leave me with a smudge on marker enzyme activity along the gradient,
ie. beta galactosidase, beta glucoronidase, and LDH. I would be most
gratful for some advice on spin times and g force required to separate these
compartments to enable accurate identification.
Thanks in advance for any suggestions.
Dept of Pharmacology
University of Western Australia
e-mail: dcarpenter at receptor.pharm.uwa.edu.au
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