RACE kit

Martyn White mbwhite at CARDINAL.NCSC.ORG
Wed Apr 6 16:58:56 EST 1994

>Subject: Re: question regarding RACE kits
>I tried the "5'-Amplifinder RACE Kit" from Clontech. It was a complete 
>failure. Even the supplied control was very poor. Other controls showed, 
>that the reverse transcription step worked. Therefore I think anchor 
>ligation using T4 RNA Ligase is the problem. Could it be that Clontech's T4 
>RNA Ligase is trash?
>Matthias Zeiner

	I had _exactly_ the same experience with this kit.  I wonder
if they have taken it off the market yet?  I understand that this
single stranded ligation is _very_ inefficient.  Caveat emptor

Martyn White

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