brunstei at UNIXG.UBC.CA
Thu Apr 7 12:24:43 EST 1994
Oops, looks like I have just put my foot (or perhaps even both!)
squarely in my mouth. The public-domain software which has given me
problems with predicted melting points for primers is 'PRIMERS' by
Anderson and Bristol from UCLA; despite these problems (variations
between predicted and observed up to 10C) I have found the software
usefull for predicting hairpins or dimers or other potential PCR
nasties. I have also used Oligo V4.1 on a Mac; I didn't have any
problems at all with it but only tried it a few times and so can't claim
to have really tested it. Oligo, by the way, is commercial software in
the $400 range, so I would hope that its melting-point predictions are
better than free public-domain or shareware code!
Sorry for any confusion this may have caused, next time I'll be
more careful to drag out the bit of software and verify names!
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