Oligo. programs

John Brunstein brunstei at UNIXG.UBC.CA
Thu Apr 7 12:24:43 EST 1994


	Oops, looks like I have just put my foot (or perhaps even both!) 
squarely in my mouth.  The public-domain software which has given me 
problems with predicted melting points for primers is 'PRIMERS' by 
Anderson and Bristol from UCLA; despite these problems (variations 
between predicted and observed up to 10C) I have found the software 
usefull for predicting hairpins or dimers or other potential PCR 
nasties.  I have also used Oligo V4.1 on a Mac; I didn't have any 
problems at all with it but only tried it a few times and so can't claim 
to have really tested it.  Oligo, by the way, is commercial software in 
the $400 range, so I would hope that its melting-point predictions are 
better than free public-domain or shareware code!
	Sorry for any confusion this may have caused, next time I'll be 
more careful to drag out the bit of software and verify names!




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