Staining PVDF (Immobilon)

Ding Ming ming at ahabs.wisc.edu
Thu Apr 7 10:47:21 EST 1994


In article <eyeballCnw7GA.Cst at netcom.com>, eyeball at netcom.com (David M.
Berman) wrote:

> I like to prestain my Western filters with Ponceau S to check protein
> loading, integrity, seperation, and transfer before I proceed with
> immunodetection.  Unfortunately, my target protein doesn't stick well
> to anything other than PVDF (it's small and greasy).  PVDF doesn't
> seem to stain well with Ponceau S using a regular acetic acid-based
> solution.  Has anybody found a better way to stain these filters?
> 
> -- 
>                                              eyeball at netcom.com

Dear eyeball,

I just went through the latest Biotechniques(vol16,4) and there is a
article about using Nile Red. I think you probably could adapt that method
in your case.

Good luck.

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Ding Ming
University of Wisconsin-Madison
Telephone: (608)265-3544     Fax: (608)262-7420
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