Staining PVDF (Immobilon)
ming at ahabs.wisc.edu
Thu Apr 7 10:47:21 EST 1994
In article <eyeballCnw7GA.Cst at netcom.com>, eyeball at netcom.com (David M.
> I like to prestain my Western filters with Ponceau S to check protein
> loading, integrity, seperation, and transfer before I proceed with
> immunodetection. Unfortunately, my target protein doesn't stick well
> to anything other than PVDF (it's small and greasy). PVDF doesn't
> seem to stain well with Ponceau S using a regular acetic acid-based
> solution. Has anybody found a better way to stain these filters?
> eyeball at netcom.com
I just went through the latest Biotechniques(vol16,4) and there is a
article about using Nile Red. I think you probably could adapt that method
in your case.
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