IF for neutrophils

Seth Corey corey at MED.PITT.EDU
Thu Apr 7 14:08:51 EST 1994


I want to do IF on human neutrophils and I need them to stick and
spread out well. We have been trying RPMI/10%FCS incubation for
30 min at 37C. Any suggestions.

Thanks,

Seth Corey, Univ of Pittsburgh



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