'Tandem' Primers for PCR Mutagenesis

Hong Dang hdang at channel.neusc.bcm.tmc.edu
Thu Apr 7 17:09:05 EST 1994


Shaun:

Would "inverse PCR" with 2 back-to-back primers, one of them mutagenic, on a
circular plasmid work for you?

With the recent progress on long distant PCR, most plasmids should be in the
feasible size range.

Hong



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