Klenow question

Henry sticknbd at ctrvax.vanderbilt.edu
Fri Apr 8 07:49:54 EST 1994


When I make random-primed probes, the protocol I use
says to stop the reaction after 30 to 60 minutes with
EDTA (or heat), which I always do.  What would be the
consequences of allowing the reaction to continue?  I
cannot envision any ill effects of letting the reaction
go, even if it were to go on for several hours.  Comments?
sticknbd at miranda.cc.vanderbilt.edu



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