chemiluminescent immunodetection

Rascals rascals at
Sat Apr 9 01:33:06 EST 1994

In article <2j3896$cr at>, jbarta at (John R Barta)

<interference from endogenous plant HRP activity>

The peroxidase activity from plants (from chlorophyll) can be eliminated on the
blots immediately after blotting by incubating the blots with hydrogen peroxide
(add about 5 mL of H2O2 to 100 mL of wash solution). The H2O2 will destroy the
peroxidase activity.

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