Klenow question

Stephen R. Lasky Stephen_Lasky at brown.edu
Sat Apr 9 10:10:06 EST 1994

In article <sticknbd-080494070634 at>,
sticknbd at ctrvax.vanderbilt.edu (Henry) wrote:

> When I make random-primed probes, the protocol I use
> says to stop the reaction after 30 to 60 minutes with
> EDTA (or heat), which I always do.  What would be the
> consequences of allowing the reaction to continue?  I
> cannot envision any ill effects of letting the reaction
> go, even if it were to go on for several hours.  Comments?
> sticknbd at miranda.cc.vanderbilt.edu

When we first started using random priming back in 1986 or so, we would let
the incubation go overnight.  I'm not sure whether that was in the original
protocol or whether it was just convenient to set up our labeling the night
before and let it go.  Bottom line is that it didn't hurt the labeling.

Stephen R. Lasky, Ph.D.       Brown University/Roger Williams Medical
e-mail: Stephen_Lasky at brown.edu         LandLine: 401-456-6572
A nuclear war could ruin your whole day.

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