cell surface labeling

[T. S. Pillay]

Subject: cell surface labeling
From: MBWEN
Date: 8 Apr 94 09:42:26 GMT
In article <01HAXVTHA5EQ000OIU at ccvax.sinica.edu.tw> ,
MBWEN at CCVAX.SINICA.EDU.TW writes:
>
>Dear netters:
>I've tried to label cells with 125I using Iodobeads from Pierce. It was
>tickier than I thought. Does anyone have good experience with it or can
>suggest other better ways for cell surface labeling. Thanks in advance.
>                                                                wen

I had a similar experience-  the iodobeads tend to sink in the culture
medium if you are using monolayer cells.  I would suggest lactoperoxidase
iodination- its simpler.